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Edward Townes. The two men formalized the establishment of the Neutral Strip in writing, agreeing that neither government would attempt to assert sovereignty over the area, send troops into the neutral territory, or allow anyone not already resident to enter until international diplomacy provided a final resolution to the boundary question. In effect, the neutral ground existed outside the governance of either the United States or Spain until After the United States acquired Louisiana from France, a dispute over the exact boundaries of the Louisiana Purchase brought Spain and the United States to the brink of war. Americans flocking to the western portion of Orleans Territory, and claiming Texas as part of the purchase, threatened the sparsely populated and poorly defended Spanish province. Exacerbated by tensions over relations with Native Americans, Spanish protection of escaped slaves, and the interruption of commercial ties between Natchitoches and Nacogdoches, each nation braced for conflict, rushing men to the border in the belief that invasion from the other was imminent.
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Stripping and Reprobing Western Blots

Strip western
Strip western
Strip western
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Get your stripping stripes! Find out how to strip and re-blot your Western - Bitesize Bio

Your browser does not have JavaScript enabled and some parts of this website will not work without it. For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome. Our Cookie Policy explains how you can opt-out of the cookies we use. Print this protocol. Stripping is the term used to describe the removal of primary and secondary antibodies from a western blot membrane.
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The Neutral Strip

While some may lump the culture of the very western portion of Louisiana—south of Natchitoches and north of Lake Charles—into the general culture of North Louisiana, others identify it as a separate folk region. Known as Louisiana's Neutral Strip, the region once separated Spanish Texas from French Louisiana and is now home to a distinct culture. In addition, folklorists, historians, and other scholars identify the region as culturally distinct and possessing many folk groups and traditions that set it apart from its neighboring regions.
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The songbird is threatened with extinction as its streamside habitat has dried up from agricultural water withdrawals and development of its streamside homes. But livestock and mining interests in Arizona and an extreme property-rights group in Texas pushed the U. The Trump administration should protect their nesting grounds, not abandon them to polluters.
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